Use the incinerator to sterilize the loop. Attach burner to gas source. Gram Staining Principle Reagents Procedure Steps Results Heat loop until entire wire glows red. . This video shows a student improperly sterilizing an inoculating loop. Part C Arrange the steps of loop sterilization in their correct order. Cool loop A student properly flames her loop but then forgets to let it cool. Methods of sterilization of surgical instruments are Boiling Incineration Autoclave. Pick up a tube of sterile nutrient broth with your free hand carefully remove the cap cotton plug with your little finger of the hand holding the loop and flame the mouth of the tube. Methods of sterilization of water we use filtration and. Dip the loop into the broth culture containing the mixture of bacteria. Lift the lid of the plate just enough to insert the loop. Light burner with striker 4. Attach burner to gas source...
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